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The column at the far right uses the second first difference as the base, which I now think is correct. The column second from the right uses the first first difference at the base.
 
Just to be clear, for layer 2 the first difference is 40 – 0 = 40. For layer 3 the first difference is 75 – 40 = 35. Therefore, for layer 2, the second difference is 35 – 40 = -5. I think this is what you would call the “middle second difference”. It tells how sharply the slope falls after the current layer, which is what we want.
 
To correct for scaling, we need to divide by a first difference. In the first concavity column, for layer 2 I use 5/40 = 0.125. For the last column for layer 2 I use 5/35 = 0.143.
Both approaches have a local max at layer 4, which is what we want. However, the second column from the right has a global max at the last layer, which is certainly not what we want. But is can happen at the end where the increments are very small.
So it seems pretty clear that we want to use the second first difference at the base. More precisely, to get the concavity for layer 3 we want to divide the middle second difference by the forward first difference. (It would probably also be okay to use the middle second difference divided by the middle first difference, but I have not checked that out).
So it seems pretty clear that we want to use the second first difference at the base. More precisely, to get the concavity for layer 3 we want to divide the middle second difference by the forward first difference. (It would probably also be okay to use the middle second difference divided by the middle first difference, but I have not checked that out).
===Version 3.5 build notes===

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